NOTE ON THE DETERMINATION OF UREA IN URINE BY DIRECT NESSLERIZATION
نویسندگان
چکیده
منابع مشابه
Direct fluorometric determination of urea in urine.
Urea in biological fluids has been determined by a variety of direct and indirect procedures. Some indirect methods depend on the hydrolysis of urea with the enzyme urease to form ammonia, with subsequent quantitation of the ammonia by nesslerization (1-4). Undesirable features of these indirect methods are the use of relatively unstable reagents and loss of ammonia during the procedure. Direct...
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The determination of urea nitrogen in blood filtrates is one of the most troublesome procedures encountered in the hospital laboratory. When the method used is that of distillation as recommended by Folin it is often necessary to repeat determinations because the solution has sucked back or frothed over. The results obtained by aeration, unless very carefully checked, are too often low and the ...
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Methods for the determination of urea in biological fluids have passed through an interesting cycle (cf. Peters and Van Slyke (3) pp. 539-544). The earliest procedures were strictly chemical ones, involving precipitation of the urea as an insoluble mercury complex or decomposition of the urea by means of heat or hypobromite. The use of the highly specific enzyme urease occurring in the jack and...
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Marshall’s1 urease method, in the modification proposed by Van Slyke and Cullen, is apparently the most accurate method available for the determination of urea. The method has, however, not yet attained the maximal degree of accuracy to be expected from volumetric processes in general. From the published figures of Van Slyke and Cullen, their modification appears to yield duplicates agreeing wi...
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The method outlined in the foregoing. paper for the estimation of urea in blood is perfectly reliable for larger quantities of urea (up to 25 mgms. or perhaps more). Its adaptation to the determination of urea in urine using only a small amount of the urine is shown in this paper. The method to be described is slightly more accurate than the rapid clinical method proposed earlier.’ The reason f...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1919
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)87379-3